Technical library - RESOURCES

Induction of differentiation:

Grow the cells in Preadipocyte Growth Medium (C-27410) until they reach complete confluence; this roughly takes 1 week. Change medium 2-3x per week.
Aspirate the Growth Medium; add Preadipocyte Differentiation Medium (C-27437) for 72 h
Aspirate the Differentiation Medium, add Adipocyte Nutrition Medium (C-27438).

The cells will now start to accumulate droplets of lipids which can be visualized under the microscope. The process usually takes 1-2 weeks. Change medium 2-3x per week. It is possible to maintain the differentiated adipocytes in Nutrition Medium for up to 4 weeks. However, the cells tend to lyse and detach after about 3 weeks.

Please note: We recommend to perform differentiation experiments at population doubling numbers lower than 4-5 (a maximum of 1 passage after thawing) in order to obtain a high differentiation level of the culture.

Our juvenile HDMEC (C-12210) are isolated from foreskin of young male donors (1-10 years). In contrast, adult HDMEC (C-12212) are derived from different skin localisations like the cheek, temple, or breast. The donors are > 20 years old and are mostly female. Adult HDMEC are the cells of choice when you need cells from a particular part of the body (other than foreskin), or if it is important for your study to use cells from female and/or adult donors.

a) HNEpC (Human Nasal Epithelial Cells) are isolated from nasal mucosa b) HTEpC (Human Tracheal Epithelial Cells) from the surface epithelium of trachea c) HBEpC from the surface epithelium of bronchie, and d) HSAEpC (Human Small Airway Epithelial Cells) from the distal portion of the respiratory tract in the 1 mm bronchiole area (comprising the cells from bronchioli and alveoli).

Our Chondrocyte Growth Medium consists of an optimized basal media formulation and is supplemented with 10 % (v/v) fetal calf serum that has undergone stringent biological controls.

Our Osteoblast Supplement consists of FCS (10 % [v/v] final concentration) which is specifically tested to support optimal growth of Normal Human Osteoblasts.

We get 5-8 new lots of adult MNC (C-12907) every month (> 15 vials each). Cord blood MNC (C-12901) are smaller lots (5-10 vials), we get 2-4 new lots every month.

Our Nasal Epithelial Cells are isolated from nasal septum or adenoids.

Yes, but remember to thaw and seed the cells in our growth factor containing Airway Epithelial Cell Growth Medium as the cells need to expand and proliferate for some days.

Always use a seeding density of 150.000 cells/cm², even if you plate the cells on transwells directly after thawing. Do not forget to coat the inserts with 30 µg/ml Collagen Type I solution (e.g., Corning Inc^®., product number 354236) before you seed the cells.

Using the product lot number, the Supporting Documents can be downloaded at: promocell.com/eq-certificates/.

The Material Safety Data Sheet (MSDS) can be directly downloaded from our website on the product page.

The PromoCell Basal Media must be stored between 4-8°C and should not be frozen, as this can lead to precipitations. The same is true after addition of the supplements: the complete medium has to be kept at 4-8°C. If you prefer to make up smaller volumes of complete medium, you can aliquot the Supplement Mix and refreeze those aliquots at -20°C until use. This way you can extend the period in which you can use the supplemented media.

The majority of our skin tissue donors are caucasians. But occasionally we also get skin biopsies from asian and black donors.

Please contact our Scientific Support if you need cells from a particular phototype or origin. They will check our inventory and send you a list of available cell lots.

We recommend a seeding density for chondrocytes between 10,000 and 20,000 cells/cm². This means that a subconfluent T25-flask with approx. 900,000 cells/T25 flask (36,000 cells/cm² ) may be either split into 3 new T25 or seeded in one T75 flask or in one 100 mm petri dish. We do not recommend a specific type or brand for the culture of HCH.