Does PromoCell recommend a specific negative control for cytokine release in activated macrophages?
For cytokine release studies, non-activated (polarized) macrophages are the recommended negative control.
Because macrophages constitutively secrete certain cytokines, the effects of M1 or M2 activation should always be assessed relative to a corresponding non-activated M(-) control. To ensure meaningful comparisons, the control cells should be cultured and handled under the same conditions as the activated macrophages, with the activation stimulus being the only difference.
Because macrophages constitutively secrete certain cytokines, the effects of M1 or M2 activation should always be assessed relative to a corresponding non-activated M(-) control. To ensure meaningful comparisons, the control cells should be cultured and handled under the same conditions as the activated macrophages, with the activation stimulus being the only difference.
Products relevant to this topic
Human M1 Macrophages (GM-CSF), Monocyte-derived, single donor, 1,5 Mio cells
Cat No
C-12914
Monocyte-derived, polarized, non-activated, primary human macrophages. Polarization in M1-Macrophage Generation Medium XF (incl. GM-CSF).
Human M2 Macrophages (M-CSF), Monocyte-derived, single donor, 1,5 Mio cells
Cat No
C-12915
Monocyte-derived, polarized, non-activated, primary human macrophages. Polarization in M2-Macrophage Generation Medium XF (incl. M-CSF).
M1-Macrophage Generation Medium XF
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C-28055
Serum-free and xeno-free medium for generation of M1 macrophages from fresh monocytes.
M2-Macrophage Generation Medium XF
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C-28056
Serum-free and xeno-free medium for generation of M2 macrophages from fresh monocytes.