Application Notes
Items 1-12 of 39 Results
-
Neurosphere culture of brain tumor stem cells
Using 3D cultures of brain cancer cells as neurospheres in serum-free conditions maintains the properties of primary tumors. In vitro models with the 3D Tumorsphere Medium XF are an essential prerequisite for research aimed at identifying ways to eradicate brain tumor cancer stem cells.
Application Notes
-
Endothelial cell transmigration and invasion assay
Angiogenesis, the development of blood vessels from existing ones, is an essential process. Common in vitro models are the transwell migration assay and the cell invasion assay.
Application Notes
-
Monocyte-derived macrophages: Customized in vitro large-scale differentiation from human PBMC
Primary human macrophages are difficult to isolate and do not proliferate in culture. We offer a complete system for easy and cost-efficient generation of pure monocyte-derived macrophages.
Application Notes
-
Endothelial cell tube formation assay (in vitro angiogenesis assay)
Different processes lead to formation of new blood vessels in the human body. The in vitro tube formation assay allows researchers to study such mechanisms in cell culture.
Application Notes
-
High-yield isolation of HUVECs in Endothelial Cell Growth Medium 2
Here we show a procedure for a high-yield isolation of primary HUVECs in PromoCell’s Endothelial Cell Growth Medium 2 (ECGM2) and the comparison with a competitor medium.
Application Notes
-
Epi-Fluorescence mitochondrial imaging in live single cells and tumorspheres
Mitochondria are the organelles that fuel life, which makes them extremely important for research. We offer a special dye for live-cell and fixed-cell imaging. Use this dye to boost your mitochondria research. Find out how.
Application Notes
-
Holotomography-monitored phagocytosis assay of M1 macrophages
Phagocytosis is an essential process for pathogen clearance and is the subject of research for many immunologists. Find out more about PromoCell's phagocytosis assay for fluorescence microscopy and how you can implement this method in your lab.
Application Notes
-
Cell death and proliferation of pharmacologically relevant human primary cells with drug treatment
Cytotoxic effects of a drug can be a crucial factor that differentiates a blockbuster from a failure. We assessed cytotoxic sensitivity of various primary cells in our portfolio using a high throughput-suitable assay.
Application Notes
-
Cancer stem cell selection from cancer cultures
Traditional culture systems for cancer cells all share a lack of specific growth support for malignant cells. PromoCell's Primary Cancer Culture System is a reliable tool for depleting stromal cells, fibroblasts, and all other types of non-cancerous cells.
Application Notes
-
Unselective cell isolation from solid human tumor and xenograft biopsies
Do your primary cells from solid human tumors and xenografts need broad growth support? Then find out how to employ the serum-free, non-selective Cancer Cell Line Medium XF.
Application Notes
-
Standardized culture of cancer cell lines under serumand xeno-free conditions
Undefined media supplements have unwanted effects on cell cultures. Learn how to obtain more accurate and comparable results with PromoCell’s Cancer Cell Line Medium XF.
Application Notes
-
Isolation of patient-derived primary cancer cells
Isolate tumor cells from patient samples and transform them into long-term cell cultures. Learn more about the primary cancer culture system and how it compares to other established culture methods.
Application Notes