My Normal Human Cells are growing slowly after subculture. What could be the reason?
Slow growth after subculture is often caused by over‑trypsinization, which can damage the cells and reduce their viability.
In addition, suboptimal culture or handling conditions may also affect growth, for example:
In addition, suboptimal culture or handling conditions may also affect growth, for example:
- Insufficient inactivation of trypsin
- EDTA not removed by centrifugation after detachment
- Incorrect seeding density
- Use of non‑recommended media or supplements
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Primary Human Keratinocytes isolated in serum-free and BPE-free Keratinocyte Growth Medium 3. Available from juvenile foreskin or adult skin from single or pooled donors.