Which endothelial cell type is recommended for in vitro angiogenesis studies?
For routine in vitro angiogenesis assays, Human Umbilical Vein Endothelial Cells (HUVEC) are the most commonly used endothelial cell type due to their availability, robust growth characteristics, and reliable performance in assays such as tube formation, migration, and sprouting assays.
However, angiogenesis in vivo primarily involves the formation of new capillaries from the microvascular endothelium. Therefore, Human Dermal Microvascular Endothelial Cells (HDMEC) and other primary human microvascular endothelial cells may provide a more physiologically relevant model for studying angiogenesis.
Compared with HUVEC, microvascular endothelial cells more closely represent the small-vessel compartment involved in tissue vascularization and may therefore yield more biologically relevant responses to angiogenic stimuli.
Recommendation:
However, angiogenesis in vivo primarily involves the formation of new capillaries from the microvascular endothelium. Therefore, Human Dermal Microvascular Endothelial Cells (HDMEC) and other primary human microvascular endothelial cells may provide a more physiologically relevant model for studying angiogenesis.
Compared with HUVEC, microvascular endothelial cells more closely represent the small-vessel compartment involved in tissue vascularization and may therefore yield more biologically relevant responses to angiogenic stimuli.
Recommendation:
- HUVEC are well suited for routine screening, assay development, and comparative studies.
- HDMEC or other microvascular endothelial cells are recommended when a physiologically relevant model of capillary angiogenesis is required.
Products relevant to this topic
Human Umbilical Vein Endothelial Cells (HUVEC) single donor
Cat No
C-12200
Primary Human Umbilical Vein Endothelial Cells isolated from the vein of the umbilical cord of single donor.
Human Umbilical Vein Endothelial Cells (HUVEC) pooled
Cat No
C-12203
Primary Human Umbilical Vein Endothelial Cells isolated from the vein of the umbilical cord of pooled donors.
Human Umbilical Vein Endothelial Cells (HUVEC) pre-screened
Cat No
C-12205
Primary Human Umbilical Vein Endothelial Cells isolated from the vein of the umbilical cord of pre-screened donors.
Human Umbilical Vein Endothelial Cells (HUVEC) isolated in Growth Medium 2, single donor
Cat No
C-12206
HUVEC isolated from the umbilical vein of single, pooled or pre-screened donors in Endothelial Cell Growth Medium 2 containing additional VEGF.
Human Umbilical Vein Endothelial Cells (HUVEC) isolated in Growth Medium 2, pooled
Cat No
C-12208
HUVEC isolated from the umbilical vein of single, pooled or pre-screened donors in Endothelial Cell Growth Medium 2 containing additional VEGF.
Human Dermal Microvascular Endothelial Cells (HDMEC) juvenile foreskin
Cat No
C-12210
Primary Human Dermal Microvascular Endothelial Cells isolated from the dermis of juvenile foreskin and adult skin.
Human Dermal Blood Endothelial Cells (HDBEC) juvenile foreskin
Cat No
C-12211
Primary Human Dermal Blood Endothelial Cells isolated from different sources (e.g. juvenile foreskin). CD31 and Dil-Ac-LDL uptake positive, Podoplanin negative.
Human Dermal Microvascular Endothelial Cells (HDMEC) adult donor
Cat No
C-12212
Primary Human Dermal Microvascular Endothelial Cells isolated from the dermis of adult skin.