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My cells are contaminated. Where does the contamination come from?

Microbial contamination is typically introduced during cell culture handling and may originate from several common sources.
 
Typical causes include:
  • Unsterile working techniques (e.g. insufficient disinfection, improper aseptic handling)
  • Contaminated equipment or environment such as:
    • Water in water baths
    • Incubators
    • Laminar flow hoods
  • Contaminated reagents or consumables including:
    • Culture media or supplements
    • PBS, trypsin, or other solutions
    • Cell culture plasticware
Microbial contamination can severely affect cell growth and morphology.

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Normal Human Epidermal Keratinocytes (NHEK) juvenile foreskin, single donor
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Primary Human Keratinocytes isolated in serum-free and BPE-free Keratinocyte Growth Medium 3. Available from juvenile foreskin or adult skin from single or pooled donors.
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Primary Human Keratinocytes isolated in serum-free and BPE-free Keratinocyte Growth Medium 3. Available from juvenile foreskin or adult skin from single or pooled donors.
Normal Human Epidermal Keratinocytes (NHEK) juvenile foreskin, isolated in Growth Medium 3, pooled
Normal Human Epidermal Keratinocytes (NHEK) juvenile foreskin, isolated in Growth Medium 3, pooled
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Primary Human Keratinocytes isolated in serum-free and BPE-free Keratinocyte Growth Medium 3. Available from juvenile foreskin or adult skin from single or pooled donors.
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Primary Human Keratinocytes isolated in serum-free and BPE-free Keratinocyte Growth Medium 3. Available from juvenile foreskin or adult skin from single or pooled donors.

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