Does precoating culture dishes have any adverse effects on HUVEC?
Precoating culture vessels with extracellular matrix (ECM) proteins does not have adverse effects on Human Umbilical Vein Endothelial Cells (HUVEC).
However, ECM coatings such as fibronectin, collagen, or gelatin have been reported to influence cellular gene and protein expression patterns.
Recommendation
To ensure reliable and comparable results, we strongly recommend using the same culture conditions throughout an entire experimental series, including:
However, ECM coatings such as fibronectin, collagen, or gelatin have been reported to influence cellular gene and protein expression patterns.
Recommendation
To ensure reliable and comparable results, we strongly recommend using the same culture conditions throughout an entire experimental series, including:
- the same ECM coating (e.g. fibronectin, collagen, or gelatin), or
- consistently uncoated culture vessels
Products relevant to this topic
Human Umbilical Vein Endothelial Cells (HUVEC) single donor
Cat No
C-12200
Primary Human Umbilical Vein Endothelial Cells isolated from the vein of the umbilical cord of single donor.
Human Umbilical Vein Endothelial Cells (HUVEC) pooled
Cat No
C-12203
Primary Human Umbilical Vein Endothelial Cells isolated from the vein of the umbilical cord of pooled donors.
Human Umbilical Vein Endothelial Cells (HUVEC) pre-screened
Cat No
C-12205
Primary Human Umbilical Vein Endothelial Cells isolated from the vein of the umbilical cord of pre-screened donors.