What is the concentration of ECGS contained in Endothelial Cell Growth Media and Preadipocyte Growth Media?
At manufacture, ECGS (Endothelial Cell Growth Supplement) is adjusted to a protein content of 3 mg/ml.
- For Human Endothelial Cells and Microvascular Endothelial Cells, the optimal amount of ECGS is 2 ml ECGS per 500 ml medium (0.4% v/v), corresponding to 6 mg extracted protein per 500 ml medium.
- In the SupplementPack and Medium Kit, ECGS is supplied together with heparin (ECGS/H) in a single vial. The ECGS/H supplement contains 22.5 mg/ml heparin and provides a final heparin concentration of 45 mg per 500 ml medium (90 µg/ml) when added as recommended.
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