Technical library - RESOURCES

Yes, our NHEM.f will also grow in our optimized Melanocyte Growth Medium M3 (C-24310) and can achieve > 15 population doublings.

Yes; the NHEK-GM2 (C-12001; C-12003; C-12005; C-12006) also grow in PromoCell Keratinocyte Growth Medium 3 (C-20021). Using the protocol with the fixed intervals; they grow slightly faster than in Keratinocyte GM2 and proliferate for > 15 PDs. Conversely; NHEK-GM3 (C-12011; C-12013; C-12015; C-12016) also grow in the existing Keratinocyte Growth Medium 2 (C-20011). When using the classical subcultivation protocol (density > 70% - 90%); they grow slightly slower compared to Keratinocyte GM3; but also reach > 15 PDs.

The NHEK (primary human keratinocytes) are isolated in our serum-free Keratinocyte Growth Medium 2 (C-20011) the NHEK GM3 in our improved serum-free and BPE-free Keratinocyte Growth Medium 3 (C-20021). Both NHEK and NHEK GM3 are available from single or from pooled donors isolated from the epidermis of juvenile foreskin or adult skin.

Yes it is possible to aliquot the 5 ml Cytokine Mix E (C-39891) into 5 x 1 ml.

The activation of macrophages as such is complete after 24 hrs. However; to maintain the activation status over a longer period of time (i.e.; several days); fresh activation factors should be added with every medium change.

The basis for the intended use of our products is defined in our Terms & Conditions under the chapter “Use of Goods”.

Our HPAEC and HPASMC are isolated from the proximal pulmonary artery.

You can also use Oil Red O to stain lipid droplets. At PromoCell; we used to use Oil Red O as well; but switched to Sudan III some time ago for organizational reasons.

Light flocculation may be seen upon thawing the supplements containing ECGS/heparin or BPE. This does not affect the activity of our media. Optionally; the precipitate can be removed by centrifugation under sterile conditions. We recommend to thaw the supplements (SupplementMix or SupplementPack) at 15-25°C.

Yes; there are a few differences: - We recommend replacing the MSC Growth Medium XF (C-28019) 3-4 h after seeding; as opposed to 16-24 hours after seeding for most other cell types/growth media. - When MSC Growth Medium XF; MSC Neurogenic Differentiation Medium; MSC Adipogenic Differentiation Medium 2 or MSC Osteogenic Differentiation Medium are used; flasks have to be coated with 10 µg/cm² (human or bovine) fibronectin according to the instruction manual. - We strongly recommend using Accutase (C-41310) for cell detachment instead of Trypsin. If Trypsin is used; contact time should not exceed 2 min.

The supplement should be at room temperature when added to the MSC Adipogenic Basal Medium 2. It may also be beneficial to invert the tube a few times to bring precipitates back into solution. Please note: It is not recommended to filter the basal medium; supplements; or complete medium; as components that induce or promote differentiation may be removed; resulting in a low differentiation rate when using the medium.

According to the product manual Cryo-SFM should be stored at 4-8°C. However since this solution is used to freeze cells in liquid nitrogen we assume that storing Cryo-SFM once at -20°C should not have a negative impact on the product quality. After thawing please store it at 4-8°C as recommended.