Technical library - RESOURCES

The PromoCell trypsin 0.04% / EDTA 0.03% solution and the PromoCell TNS solution are both based on HepesBSS. Therefore, it is best to also use HepesBSS to wash the cells prior to trypsinization.

We recommend to use the DetachKit when subculturing our Normal Human Cells. It contains a HepesBSS washing buffer, trypsin 0.04% / EDTA 0.03% solution, and TNS, a trypsin inhibitor from soybean. Please note: Many of our culture media have low serum content or no serum at all. These media are not suitable to inactivate trypsin during subculture.

The population doubling time or generation time (t_g) is usually calculated during the logarithmic phase of growth. It specifies the time (t) in hours needed by the culture to double its cell number.

t_g = t / n

t = time (hrs) between 2 cell counts

n= number of population doublings

The Certificates of Analysis can easily be downloaded from our CoA search page. Simply type in the lot number indicated on the cryovial/TC-flask and click the SEARCH button.

You need to have experience working under sterile conditions and under a laminar flow hood. It is of advantage to have experience with other cell types and/or cell lines. If you are a beginner in cell culture and would like to establish a cell culture lab, we will assist you in working with PromoCell Normal Human Cells.

Serum turbidity is usually caused by cryoprecipitation of lipid components during freezing and thawing. The more times the serum is subjected to freeze/thaw cycles, the more turbidity is noticed.

It can be minimized by freezing the serum in aliquots at -20°C and thawing these aliquots individually at the time of use.

Differentiation of hMSC into mature adipocytes takes approx. 2 weeks. You can keep the adipocytes for up to 3 weeks in the MSC Adipogenic Differentiation Medium. After 3 weeks we recommend to switch to PromoCell's Adipocyte Nutrition Medium (C-27438).

Changing the medium every 2-3 days, the neuronally differentiated MSC can be kept in culture for up to 2 weeks.

Human Mesenchymal Stem Cells have retained the ability to differentiate into a variety of cell types including fat cells, chondrocytes, and osteoblasts. PromoCell supply hMSC from 3 different tissues: bone marrow (hMSC-BM; C-12974), umbilical cord matrix (hMSC-UC; C-12971), and adipose tissue (hMSC-AT; C-12977).

MSC-BM show very good differentiation into bone cells but also into chondrocytes and fat cells when the respective Differentiation Media is used.
MSC-UC have a high potential to differentiate into chondrocytes but only weak potential for fat or bone cell differentiation.
In contrast, MSC-AT differentiate very well into fat and bone cells but only moderately into chondrocytes.

In other words, to obtain a high percentage of bone cells, MSC-BM or MSC-AT are the cells of choice. There are of course lot-to-lot variations and the differentiation will decrease from passage to passage. If you need MSCs with a particularly high osteoblast differentiation capacity, you can contact our Scientific Support before placing your order so that we can select an appropriate cell lot for you.

The population doubling times for PromoCell hMSC (hMSC-BM, hMSC-AT, hMSC-UC) are typically ≤ 30 hrs when using Mesenchymal Stem Cell Growth Medium 2 (C-28009). If you seed the hMSC at 4.000 cells/cm², it will take between 4-7 days until they reach subconfluency.

Our MNC lots are checked for the rate of lymphocytes, monocytes and granulocytes w/o staining by FSC/SSC using a flow cytometer. A sample plot is attached. For our purified CD14⁺ monocytes we additionally perform a CD14⁺ staining.

It takes 7-8 days to generate fully mature myeloid dendritic cells.