Technical Library
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There are two options for isolating RNA from cells stored in RNAlater Solution:
1) The solution is removed from the cells prior to extraction by centrifugation at 5;000 x g for 10 minutes at 4°C.
Note: Because of the density of RNAlater® solution; greater centrifugal forces are required to spin down the cells.
2) If no pellet is visible after centrifugation; RNA can also be purified directly from the RNAlater® solution. This can be done by adding 2 ml of 10x lysis buffer; and proceeding normally.
PromoCell cell pellets (C-14**) are an easily accessible source of DNA RNA and proteins.
No the PromoCell cell pellets (C-14**) are frozen at -20°C and cannot be revived. Their main application is to analyze RNA or protein. Cryopreserved cells that can be revived are available from the same donors. Please contact our Technical Customer Support if you need matched viable cells.
Cell type-specific markers are usually determined one passage after thawing.
Example: After thawing; HUVEC are in P1. Markers (CD31 expression; Dil-Ac-LDL uptake) are tested in P2.
Please note: Cells that are frozen directly after isolation (blood cells) are tested directly after thawing with no further culturing step.
a) HNEpC (Human Nasal Epithelial Cells) are isolated from nasal mucosa
b) HTEpC (Human Tracheal Epithelial Cells) from the surface epithelium of trachea
c) HBEpC from the surface epithelium of bronchie; and
d) HSAEpC (Human Small Airway Epithelial Cells) from the distal portion of the respiratory tract in the 1 mm bronchiole area
(comprising the cells from bronchioli and alveoli).
- HDLEC and HDBEC both express the typical endothelial cell marker CD31 (= PECAM-1).
- HDLEC cultures are additionally tested positive for podoplanin; a transmembrane glycoprotein involved in lymphatic vessel formation; whereas HDBECs are podoplanin-negative.
Our HUtMEC are isolated from the middle layer of the uterine wall (myometrium). The tissue donors were not pre-treated with hormones.
Our HPMEC are isolated from peripheral lung tissue of adult donors.
Juvenile HDMEC (C-12210) are isolated from the dermis of foreskin. Adult HDMEC (C-12212) are isolated from different regions. The localizations include cheek temple breast upper arm and labia. Please contact our Technical Customer Support if you need HDMEC from a particular part of the body.
Our Chondrocyte Growth Medium consists of an optimized basal media formulation and is supplemented with 10 % (v/v) fetal calf serum that has undergone stringent biological controls.
PromoCell cell pellets (C-14**) can be stored indefinitely at -20°C and are stable for up to 1 month at 4°C and up to one week at room temperature.
Please note: The samples do not freeze at -20°C.
The passage number varies depending on the cell type. Please refer to the Manual for your cells under "Specifications".
You will also find the information in the Certificate of Analysis (CoA).