Is it normal that CD34⁺ progenitor cells appear to attach to tissue culture plastic after a week‑long culture in HPC Expansion Medium XF (C‑28021)?
Yes, this observation is normal and does not indicate true cell attachment.
CD34⁺ progenitor cells cultured in HPC Expansion Medium XF (C‑28021) are non‑adherent cells. During culture, they may sink to the bottom of the culture vessel, which can give the impression that they have attached.
However:
CD34⁺ progenitor cells cultured in HPC Expansion Medium XF (C‑28021) are non‑adherent cells. During culture, they may sink to the bottom of the culture vessel, which can give the impression that they have attached.
However:
- The cells do not firmly attach to the tissue culture plastic
- They retain a round morphology
- They can be easily rinsed off with culture medium
