How long does it take to grow chondrocytes to subconfluence, and how many doublings do they undergo per passage?
Under standard culture conditions, PromoCell Normal Human Chondrocytes (HCH, C‑12710) typically reach subconfluency within ~4-7 days after seeding.
The number of population doublings (PDs) per passage depends on the seeding density and the cell yield at subconfluency and can be calculated accordingly.
As a general guideline:
When HCH are seeded at 10,000 cells/cm², they typically undergo approximately 2 population doublings per passage.
The number of population doublings (PDs) per passage depends on the seeding density and the cell yield at subconfluency and can be calculated accordingly.
As a general guideline:
When HCH are seeded at 10,000 cells/cm², they typically undergo approximately 2 population doublings per passage.
