Can I cultivate macrophages in complete medium without cytokines?
No. Macrophages require cytokine support for survival.
Without cytokines, macrophages will die very quickly, even when cultured in otherwise complete medium. To maintain viability, Macrophage Base Medium XF (basal medium + SupplementMix) must be supplemented with:
Without cytokines, macrophages will die very quickly, even when cultured in otherwise complete medium. To maintain viability, Macrophage Base Medium XF (basal medium + SupplementMix) must be supplemented with:
- the appropriate cytokines (e.g. GM‑CSF or M‑CSF, depending on macrophage type), or
- at least human AB serum as a minimal survival support.
Products relevant to this topic
M1-Macrophage Generation Medium XF
Cat No
C-28055
Serum-free and xeno-free medium for generation of M1 macrophages from fresh monocytes.
M2-Macrophage Generation Medium XF
Cat No
C-28056
Serum-free and xeno-free medium for generation of M2 macrophages from fresh monocytes.
Macrophage Base Medium XF
Cat No
C-28057
User-customizable, serum-free and xeno-free medium for generation of macrophages from fresh monocytes. Medium does not include cytokines.
Human M1 Macrophages (GM-CSF), Monocyte-derived, single donor, 1,5 Mio cells
Cat No
C-12914
Monocyte-derived, polarized, non-activated, primary human macrophages. Polarization in M1-Macrophage Generation Medium XF (incl. GM-CSF).
Human M2 Macrophages (M-CSF), Monocyte-derived, single donor, 1,5 Mio cells
Cat No
C-12915
Monocyte-derived, polarized, non-activated, primary human macrophages. Polarization in M2-Macrophage Generation Medium XF (incl. M-CSF).