I would like to isolate TAM from tumor tissue. Which medium should I use?

Tumor-associated macrophages (TAM) represent an important constituent of the tumor microenvironment. After being educated by cancer cells, TAM adopt an anti-inflammatory, pro-tumor and pro-metastatic M2-like phenotype.

Our Primary Cancer Cell Medium D-ACF allows for the isolation and culture of tumor associated macrophages (TAM) from solid tumors while reliably preventing stromal overgrowth.

The isolated TAM are non-proliferating adherent cells and can be cultured for at least two weeks.