How can RNA best be isolated from PromoCell cell pellets (C‑14***)?

Q: How can RNA best be isolated from PromoCell cell pellets (C‑14***)?

PromoCell cell pellets (C‑14**) are supplied in RNAlater TM Solution, which allows for two established approaches to RNA isolation. Option 1 : Remove RNAlater TM prior to RNA extraction Centrifuge the sample at 5,000×g for 10 minutes at 4 °C. Carefully remove the supernatant (RNAlater TM ). Proceed with RNA extraction using your standard protocol. Note: Due to the high density of RNAlater TM , higher centrifugal forces are required to efficiently pellet the cells. Option 2 : Direct RNA isolation from RNAlater TM If no visible cell pellet forms after centrifugation, RNA can also be isolated directly from the RNAlater TM solution: Add 2 ml of 10× lysis buffer directly to the sample. Proceed with RNA purification according to your standard RNA isolation protocol.

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Accepted Answer

PromoCell cell pellets (C‑14**) are supplied in RNAlater^TM Solution, which allows for two established approaches to RNA isolation.

Option 1: Remove RNAlater^TM prior to RNA extraction

Centrifuge the sample at 5,000×g for 10 minutes at 4 °C.
Carefully remove the supernatant (RNAlater^TM).
Proceed with RNA extraction using your standard protocol.

Note: Due to the high density of RNAlater^TM, higher centrifugal forces are required to efficiently pellet the cells.

Option 2: Direct RNA isolation from RNAlater^TM
If no visible cell pellet forms after centrifugation, RNA can also be isolated directly from the RNAlater^TM solution:

Add 2 ml of 10× lysis buffer directly to the sample.
Proceed with RNA purification according to your standard RNA isolation protocol.

Quote Summary

How can RNA best be isolated from PromoCell cell pellets (C‑14***)?

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