Is it possible to use SkMC (C‑12530) without the Differentiation Medium?
Yes, SkMC (C‑12530) can be cultured in Skeletal Muscle Cell Growth Medium (C‑23060) without using differentiation medium. Under these conditions, the cells remain as proliferating myoblasts.
These proliferating cells can be used for studies focusing on the induction or inhibition of cell proliferation.
However, to study the function of differentiated skeletal muscle cells (multinucleated myotubes), it is necessary to switch to Differentiation Medium (C‑23061) once the cells reach 60–80% confluency.
A detailed differentiation protocol is provided in the Instruction Manual for SkMC media.
These proliferating cells can be used for studies focusing on the induction or inhibition of cell proliferation.
However, to study the function of differentiated skeletal muscle cells (multinucleated myotubes), it is necessary to switch to Differentiation Medium (C‑23061) once the cells reach 60–80% confluency.
A detailed differentiation protocol is provided in the Instruction Manual for SkMC media.
Products relevant to this topic
Human Skeletal Muscle Cells (SkMC)
Cat No
C-12530
Primary Human Skeletal Muscle Cells isolated from different skeletal muscles from adult single donors.
Skeletal Muscle Cell Growth Medium (Ready-to-use)
Cat No
C-23060
Ready-to-use low-serum cell culture medium for growth of skeletal muscle cells.
Skeletal Muscle Cell Differentiation Medium (Ready-to-use)
Cat No
C-23061
Defined and animal-component free cell culture medium for differentiation of skeletal muscle cells.