How does PromoCell identify monocytes in hMNC samples for the Certificate of Analysis?
For human mononuclear cell (hMNC-PB) lots, PromoCell determines the proportions of the main cell populations (lymphocytes, monocytes, granulocytes) using flow cytometry without antibody staining.
Method used for hMNC lots
Additional QC for purified monocytes
For purified CD14⁺ monocytes, PromoCell performs additional antibody staining for CD14 and confirms purity by flow cytometry.
Method used for hMNC lots
- Cell populations are distinguished by forward scatter (FSC) and side scatter (SSC) characteristics in a flow cytometer.
- FSC reflects cell size, while SSC reflects cellular granularity/complexity.
- Based on these physical properties, lymphocytes, monocytes, and granulocytes form distinct clusters, allowing estimation of their relative percentages without staining.
Additional QC for purified monocytes
For purified CD14⁺ monocytes, PromoCell performs additional antibody staining for CD14 and confirms purity by flow cytometry.
Products relevant to this topic
Human Mononuclear Cells from Peripheral Blood (hMNC-PB), single donor, ultra-pure
Cat No
C-12907
Primary Human Mononuclear Cells isolated from umbilical cord blood and peripheral blood.
Human CD14+ Monocytes (hMoCD14+-PB)
Cat No
C-12909
Human CD14+ Monocytes isolated from peripheral blood (hMoCD14+-PB) of single donors.