Does the differentiation capacity of human mesenchymal stem cells change over time? When is the best time to induce differentiation?
Yes, the differentiation capacity of human mesenchymal stem cells (hMSCs) does change over time and is influenced by the number of population doublings (PDs) the cells have undergone.
Observed differentiation capacity over time
PromoCell has tested the differentiation of hMSCs into adipocytes, chondrocytes, and osteoblasts.
Good differentiation rates are still observed after approximately 10 population doublings.
However, with increasing population doublings, the differentiation potential gradually declines.
Best practice for differentiation experiments
To achieve optimal differentiation efficiency, it is recommended to use early‑passage cells with a low number of population doublings.
Observed differentiation capacity over time
PromoCell has tested the differentiation of hMSCs into adipocytes, chondrocytes, and osteoblasts.
Good differentiation rates are still observed after approximately 10 population doublings.
However, with increasing population doublings, the differentiation potential gradually declines.
Best practice for differentiation experiments
To achieve optimal differentiation efficiency, it is recommended to use early‑passage cells with a low number of population doublings.
Products relevant to this topic
Human Mesenchymal Stem Cells from Umbilical Cord Matrix (hMSC-UC)
Cat No
C-12971
Primary Human Mesenchymal Stem Cells. Available from adipose tissue, bone marrow and umbilical cord matrix.
Human Mesenchymal Stem Cells from Bone Marrow (hMSC-BM)
Cat No
C-12974
Primary Human Mesenchymal Stem Cells. Available from adipose tissue, bone marrow and umbilical cord matrix.
Human Mesenchymal Stem Cells from Adipose Tissue (hMSC-AT)
Cat No
C-12977
Primary Human Mesenchymal Stem Cells. Available from adipose tissue, bone marrow and umbilical cord matrix.
Mesenchymal Stem Cell Chondrogenic Differentiation Medium
Cat No
C-28012
Serum-free cell culture medium for differentiation of mesenchymal cells into chondrogenic lineages.
Mesenchymal Stem Cell Osteogenic Differentiation Medium
Cat No
C-28013
Cell culture medium for differentiation of mesenchymal cells into osteogenic lineages.
Mesenchymal Stem Cell Neurogenic Differentiation Medium
Cat No
C-28015
Serum-free cell culture medium for differentiation of mesenchymal cells into neurogenic lineages.
Mesenchymal Stem Cell Adipogenic Differentiation Medium 2
Cat No
C-28016
Cell culture medium for differentiation of human mesenchymal cells into adipogenic lineages.