At what level of confluency should SkMC differentiation typically be initiated?
Best differentiation results for PromoCell skeletal muscle cells (SkMCs) are obtained when cells have reached 60-80% confluency.
Recommended differentiation workflow:
Recommended differentiation workflow:
- At 60-80% confluency, aspirate the Growth Medium.
- Replace it with Skeletal Muscle Differentiation Medium to initiate differentiation.
- After 2–8 days, formation of multinucleated syncytia (myotubes) can typically be observed.
- For stable and robust differentiation, switch back to Skeletal Muscle Cell Growth Medium after 5 days of incubation in Differentiation Medium.
Products relevant to this topic
Human Skeletal Muscle Cells (SkMC)
Cat No
C-12530
Primary Human Skeletal Muscle Cells isolated from different skeletal muscles from adult single donors.
Skeletal Muscle Cell Growth Medium (Ready-to-use)
Cat No
C-23060
Ready-to-use low-serum cell culture medium for growth of skeletal muscle cells.
Skeletal Muscle Cell Differentiation Medium (Ready-to-use)
Cat No
C-23061
Defined and animal-component free cell culture medium for differentiation of skeletal muscle cells.