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Endothelial Cell Growth Medium
Endothelial Cell Growth Medium is a low-serum (2% V/V) medium optimized for the cultivation of endothelial cells from large blood vessels.
Although all our media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for bovine, porcine, murine, caprine, rabbit, elephant, dog, and rat endothelial cells from large vessels as well as for various endothelial cell lines. View a list of references where this medium was used with other species and cell types.
Components:
- Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix
- Growth Medium Kit: Includes Basal Medium and SupplementPack
- SupplementMix: Contains all media supplements premixed in one vial
- SupplementPack: Contains all media supplements as individual vials
| Media supplement | Final supplement concentration |
|---|---|
| Fetal Calf Serum | 0.02 ml / ml |
| Endothelial Cell Growth Supplement | 0.004 ml / ml |
| Epidermal Growth Factor (recombinant human) | 0.1 ng / ml |
| Basic Fibroblast Growth Factor (recombinant human) | 1 ng / ml |
| Heparin | 90 μg / ml |
| Hydrocortisone | 1 μg / ml |
Note: For best transfection results we recommend using our heparin-free Endothelial Cell Growth Medium MV2. Heparin, included in all our other Endothelial Cell Growth Media formulations, may reduce transfection efficiency.
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