Can I thaw a vial of HBEpC and seed the cells directly onto Transwell inserts for an ALI experiment?
Yes, this is possible. However, please note the following important points.
Medium and expansion
After thawing, HBEpC must be seeded and cultured in growth factor-containing Airway Epithelial Cell Growth Medium or Growth Medium 2.
Even when cells are seeded directly onto Transwell inserts, they need to expand and proliferate for several days before the air-liquid interface (ALI) is established.
Seeding density
A seeding density of 150,000 cells/cm² is recommended for all applications, including direct seeding of freshly thawed cells into Transwell inserts.
Using a lower seeding density may impair confluence and subsequent differentiation.
Coating of Transwell inserts
Before seeding the cells, Transwell inserts must be coated with Collagen Type I (30 µg/ml).
Medium and expansion
After thawing, HBEpC must be seeded and cultured in growth factor-containing Airway Epithelial Cell Growth Medium or Growth Medium 2.
Even when cells are seeded directly onto Transwell inserts, they need to expand and proliferate for several days before the air-liquid interface (ALI) is established.
Seeding density
A seeding density of 150,000 cells/cm² is recommended for all applications, including direct seeding of freshly thawed cells into Transwell inserts.
Using a lower seeding density may impair confluence and subsequent differentiation.
Coating of Transwell inserts
Before seeding the cells, Transwell inserts must be coated with Collagen Type I (30 µg/ml).
Products relevant to this topic
Human Bronchial Epithelial Cells (HBEpC)
Cat No
C-12640
Primary Human Bronchial Epithelial Cells isolated from the surface epithelium of human bronchi.
Air-Liquid Interface Medium
Cat No
C-21080
Serum-free and BPE-free cell culture medium for an optimal and standardized culture of human bronchial epithelial cells at the air-liquid interface (ALI).
Airway Epithelial Cell Growth Medium (Ready-to-use)
Cat No
C-21060
Ready-to-use serum-freecell culture medium for epithelial cells from large air passages.
Airway Epithelial Cell Growth Medium 2
Cat No
C-21040
Serum-free and BPE-free cell culture medium for epithelial cells isolated from large air passages.