My Normal Human Cells didn’t attach after thawing. What could be the reason?
Poor or absent attachment after thawing is usually caused by stress during freezing, storage, or thawing, or by suboptimal culture or handling conditions immediately after seeding.
Common reasons include:
Common reasons include:
- Inappropriate freezing or storage conditions (e.g. incorrect freezing protocol or storage outside liquid nitrogen)
- Suboptimal thawing procedure (e.g. slow thawing, prolonged exposure to DMSO, incomplete DMSO dilution after thawing)
- Inadequate culture conditions such as incorrect or incomplete growth medium, expired or improperly stored media or supplements, incorrect incubator settings (temperature, CO₂, humidity), unsuitable cell culture plasticware or missing coating (if required).
Products relevant to this topic
Normal Human Epidermal Keratinocytes (NHEK) juvenile foreskin, single donor
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C-12001
Primary Human Keratinocytes isolated from the epidermis of juvenile foreskin or adult skin from single or pooled donors.
Normal Human Epidermal Keratinocytes (NHEK) adult, single donor
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Primary Human Keratinocytes isolated from the epidermis of juvenile foreskin or adult skin from single or pooled donors.
Normal Human Epidermal Keratinocytes (NHEK) juvenile foreskin, pooled
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Primary Human Keratinocytes isolated from the epidermis of juvenile foreskin or adult skin from single or pooled donors.
Normal Human Epidermal Keratinocytes (NHEK) adult, pooled
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Primary Human Keratinocytes isolated from the epidermis of juvenile foreskin or adult skin from single or pooled donors.
Normal Human Epidermal Keratinocytes (NHEK), juvenile foreskin isolated in Growth Medium 3, single donor
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Primary Human Keratinocytes isolated in serum-free and BPE-free Keratinocyte Growth Medium 3. Available from juvenile foreskin or adult skin from single or pooled donors.
Normal Human Epidermal Keratinocytes (NHEK) adult, isolated in Growth Medium 3, single donor
Cat No
C-12013
Primary Human Keratinocytes isolated in serum-free and BPE-free Keratinocyte Growth Medium 3. Available from juvenile foreskin or adult skin from single or pooled donors.
Normal Human Epidermal Keratinocytes (NHEK) juvenile foreskin, isolated in Growth Medium 3, pooled
Cat No
C-12015
Primary Human Keratinocytes isolated in serum-free and BPE-free Keratinocyte Growth Medium 3. Available from juvenile foreskin or adult skin from single or pooled donors.
Normal Human Epidermal Keratinocytes (NHEK) adult, isolated in Growth Medium 3, pooled
Cat No
C-12016
Primary Human Keratinocytes isolated in serum-free and BPE-free Keratinocyte Growth Medium 3. Available from juvenile foreskin or adult skin from single or pooled donors.