Technical library

The difference between juvenile and adult Human Dermal Microvascular Endothelial Cells (HDMEC) is based on donor age, tissue source, and typical application context:

• Juvenile HDMEC (C‑12210) are isolated from the foreskin of young male donors (approximately 1–10 years old). These cells represent juvenile, foreskin‑derived dermal microvascular endothelium. 
• Adult HDMEC (C‑12212) are isolated from different adult skin localizations, such as breast, abdomen, or labia. The donors are older than 20 years and are predominantly female. 

Which HDMEC should you choose?

• Use adult HDMEC (C‑12212) if your study requires:endothelial cells from a specific body site other than foreskin, or cells from adult and/or female donors.
• Use juvenile HDMEC (C‑12210) if foreskin‑derived cells are acceptable, and donor age or sex is not a critical parameter for your study.

PromoCell airway epithelial cells are isolated from defined anatomical regions of the human respiratory tract:
a) HNEpC (Human Nasal Epithelial Cells): Isolated from the nasal mucosa. 
b) HTEpC (Human Tracheal Epithelial Cells): Isolated from the surface epithelium of the trachea. 
c) HBEpC (Human Bronchial Epithelial Cells): Isolated from the surface epithelium of the bronchi. 
d) HSAEpC (Human Small Airway Epithelial Cells): Isolated from the distal portion of the respiratory tract, corresponding to the ~1 mm bronchiole region, comprising cells from the bronchioles and alveoli.

PromoCell Chondrocyte Growth Medium consists of an optimized basal medium formulation and is supplemented with 10 % (v/v) fetal calf serum (FCS/FBS).
The FBS used has undergone stringent biological quality controls to ensure reliable performance and optimal growth of human chondrocytes.

The Osteoblast Supplement (C‑39615) consists of fetal calf serum (FCS/FBS), which is added to the basal medium at a final concentration of 10 % (v/v).
The FBS used in this supplement is specifically tested and selected to support optimal growth of Normal Human Osteoblasts.

PromoCell Human Nasal Epithelial Cells (HNEpC, C‑12620) are isolated from nasal mucosal tissue, specifically from the nasal septum or the adenoids. 
These tissues are part of the upper respiratory tract and represent physiologically relevant sources of human nasal epithelial cells used in airway and mucosal research.

Yes, this is possible. However, please note the following important points.

Medium and expansion
After thawing, HBEpC must be seeded and cultured in growth factor-containing Airway Epithelial Cell Growth Medium or Growth Medium 2.
Even when cells are seeded directly onto Transwell inserts, they need to expand and proliferate for several days before the air-liquid interface (ALI) is established.

Seeding density
A seeding density of 150,000 cells/cm² is recommended for all applications, including direct seeding of freshly thawed cells into Transwell inserts.
Using a lower seeding density may impair confluence and subsequent differentiation.

Coating of Transwell inserts
Before seeding the cells, Transwell inserts must be coated with Collagen Type I (30 µg/ml).

No. PromoCell Skeletal Muscle Cell Basal Medium must not be frozen and should be stored at 4-8°C. Freezing the basal medium can lead to precipitation and loss of medium quality.
The same applies after supplement addition: the complete Skeletal Muscle Cell Growth Medium must also be stored at 4-8°C and should not be frozen.
 
Recommended approach
If you prefer to prepare smaller volumes:

• Aliquot the SkMC SupplementMix separately
• Store the aliquots at -20°C
• Thaw and add an aliquot to fresh basal medium when needed

This approach helps extend the usable lifetime of the supplemented medium while maintaining optimal performance.

Yes, to a limited extent. The majority of PromoCell skin tissue donors are Caucasian, but skin biopsies from Asian and Black donors are occasionally available.
If you require melanocytes from a specific phototype or ethnic origin, please contact PromoCell Scientific Support. They can check current inventory and provide a list of available cell lots that match your requirements.

PromoCell recommends a seeding density of 10,000-20,000 cells/cm² for the culture of human chondrocytes (HCH). The following example calculations are based on a seeding density of 10,000 cells/cm².
 
Example for subculturing
A subconfluent T25 flask typically contains approximately 900,000 cells per flask, corresponding to about 36,000 cells/cm².
Based on a target seeding density of 10,000 cells/cm², this culture can be split into three new T25 flasks or into one T75 flask (→ ~12,000 cells/cm²) or
seeded into one 100 mm petri dish → ~900,000 cells per dish (→ ~9,000-10,000 cells/cm², depending on the exact growth area).
In our QC, chondrocytes are routinely seeded at densities down to 5,000 cells/cm². Even under these conditions, robust growth is observed.
 
Plasticware recommendation
PromoCell does not recommend a specific type or brand of plasticware for culturing human chondrocytes. Standard tissue‑culture‑treated flasks or dishes are suitable.

The Chondrocyte Growth Medium SupplementMix (C‑39635) contains 10% (v/v) fetal calf serum (FCS/FBS), which has been specifically tested and qualified for the culture of primary human chondrocytes.

• FBS naturally contains a variety of growth factors.
• However, these growth factors are not analyzed or defined individually.
• No additional recombinant growth factors are added to the SupplementMix.