Can the Primary Cancer Culture System (PCCS) also be used for suspension cells, such as blood cancer cells or leukemic stem cells?
Yes. The Primary Cancer Culture System (PCCS, C‑28081) supports cancer stem cells (CSC) independent of their tissue origin. Therefore, it can also be used for CSCs derived from blood cancers.
Important modification for suspension cells
When working with suspension cells, PromoCell recommends not coating the culture vessel surface with the NCCD reagent.
Instead:
Important modification for suspension cells
When working with suspension cells, PromoCell recommends not coating the culture vessel surface with the NCCD reagent.
Instead:
- Add 12.5 µl NCCD per ml of PCCS medium directly to the culture medium.
- Seed the suspension cells directly into the supplemented PCCS medium.
