Will hematopoietic progenitor cells proliferate in Hematopoietic Progenitor Cell Expansion Medium XF without Cytokine Mix E?

No. Hematopoietic progenitor cells (e.g. CD34⁺ or CD133⁺ cells) will not proliferate in Hematopoietic Progenitor Cell Expansion Medium XF (C‑28021) without additional cytokines.
 
Required supplementation
For efficient expansion, the medium must be supplemented with either:

• Cytokine Mix E (C‑39890), or
• a user‑defined cytokine mixture with equivalent functionality.

Cytokine Mix E is a ready‑to‑use formulation containing recombinant human Thrombopoietin (TPO), Stem Cell Factor (SCF), Flt3‑Ligand, and Interleukin‑3 (IL‑3).
 
Expansion performance
When used in combination with Cytokine Mix E, Hematopoietic Progenitor Cell Expansion Medium XF supports strong progenitor cell expansion for at least two weeks.