Why are PromoCell cryopreserved CD14⁺ monocytes (C‑12909) not recommended for macrophage differentiation?

Freshly isolated monocytes readily attach when cultured in Monocyte Attachment Medium (C‑28051), whereas cryopreserved CD14⁺ monocytes (C‑12909) largely lose this attachment capability after thawing.
Monocyte attachment is important not only for establishing an adherent culture but also as a key step in the differentiation process from monocytes to macrophages.
As a result, cryopreserved CD14⁺ monocytes generally show reduced macrophage differentiation efficiency, leading to lower yields and less complete differentiation compared to freshly isolated monocytes.
For optimal macrophage generation, we therefore recommend using freshly isolated PBMC or monocytes in combination with Monocyte Attachment Medium (C‑28051).