NHEK.f are isolated from tissue samples (foreskin) of single donors; aged between 1-10 years. NHEK.f pooled are prepared from the foreskins of 3 individual donors. The cells of each donor are expanded in separate TC vessels and the cells are pooled after secondary culture; before cryopreservation.
Both NHEK from single donor and NHEK pooled are in P2 after thawing.
