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What HLA typing method does PromoCell use for its HLA-typed primary cells?

HLA typing can be performed using various DNA-based methods, including sequence-specific primers (SSP), sequence-specific oligonucleotides (SSO), and next-generation sequencing (NGS).
At PromoCell, HLA typing is performed using intermediate-resolution (4-digit) PCR-RSSO analysis. In this method, the relevant HLA loci are amplified by PCR, and the resulting products are analyzed on the Luminex® platform using sequence-specific oligonucleotide probes.
This approach provides reliable HLA typing data for applications that require donor HLA information.
 
Further information: Additional details about PromoCell's HLA-typed primary cells are available on our HLA-Typed Human Primary Cells webpage.

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