Looking at the detachment protocol of PromoCell's Macrophage Detachment Solution I observed that we need to add HSA to the PBS. Can I use FBS instead? Also why are we adding 2 mM EDTA to the PBS?
Description
- FBS (or BSA) can be substituted for HSA; however we do not recommend this as the FBS will lead to unpreferable immunologic stimulation of human macrophages.
- The addition of EDTA to the PBS is to further augment the “anti-clumping” activity of the Ca2+/Mg2+ free PBS.