In which medium can monocyte‑derived dendritic cells be maintained after differentiation is completed?
After completion of differentiation (day 7/8 of the protocol), monocyte‑derived dendritic cells (DCs) should be maintained in:
Because dendritic cells are metabolically active, the medium should be changed every 3 days.
Under these conditions, we have observed that the dendritic cell phenotype remains stable for up to 7 days after differentiation is completed.
- Complete Dendritic Cell Generation Medium or
- Dendritic Cell Generation Medium XF, including all cytokines.
Because dendritic cells are metabolically active, the medium should be changed every 3 days.
Under these conditions, we have observed that the dendritic cell phenotype remains stable for up to 7 days after differentiation is completed.
Products relevant to this topic
DC Generation Medium (Ready-to-use)
Cat No
C-28050
Cell culture medium for the generation of dendritic cells from peripheral blood monocytes.
416,00 €
DC Generation Medium XF (Ready-to-use)
Cat No
C-28052
Serum-free and xeno-free medium for the generation of dendritic cells from peripheral blood monocytes. The Base Medium XF does not include cytokines.
832,00 €
DC Base Medium
Cat No
C-28053
Cell culture medium for the generation of dendritic cells from peripheral blood monocytes.
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DC Base Medium XF
Cat No
C-28054
Serum-free and xeno-free medium for the generation of dendritic cells from peripheral blood monocytes. The Base Medium XF does not include cytokines.
230,00 €