How should the NCCD reagent be stored?
After arrival, the NCCD reagent may be aliquoted and stored at -20°C for long-term storage.
For short-term use, the unopened vial may be stored at 2-8°C for up to 3 months.
Once diluted, the NCCD working solution can be stored at 2-8°C for up to 4 weeks, protected from light.
Important notes
The NCCD reagent is supplied as a 20× stock solution and must be diluted with PBS before use.
One 2 ml vial yields 40 ml of ready-to-use solution, sufficient to treat approximately 400 cm² of culture surface (equivalent to about seven 6-well plates).
Application
Use 100 µl of diluted NCCD solution per cm² of culture surface.
Incubate the closed culture vessel for at least 1 hour at room temperature
For short-term use, the unopened vial may be stored at 2-8°C for up to 3 months.
Once diluted, the NCCD working solution can be stored at 2-8°C for up to 4 weeks, protected from light.
Important notes
The NCCD reagent is supplied as a 20× stock solution and must be diluted with PBS before use.
One 2 ml vial yields 40 ml of ready-to-use solution, sufficient to treat approximately 400 cm² of culture surface (equivalent to about seven 6-well plates).
Application
Use 100 µl of diluted NCCD solution per cm² of culture surface.
Incubate the closed culture vessel for at least 1 hour at room temperature
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