How should I use accutase for cell detachment?
Short protocol:
- Wash the cells with sterile PBS (w/o Ca++/Mg++) or HepesBSS
- Add undiluted accutase to the culture vessel (2 ml per 25 cm2)
- Incubate at room temperature for 5-15 min or at 37°C for faster detachment
- When the majority of the cells has detached, centrifuge the suspension and resuspend the pellet in fresh medium. In most cases, no additional washes or neutralization steps are required.
Products relevant to this topic
Accutase-Solution
Cat No
C-41310
Accutase-Solution, primary human cell culture tested.
73,00 €
Dulbeccos PBS, w/o Ca++ / Mg++
Cat No
C-40232
Dulbecco's PBS, ready-to-use with or without Ca²⁺ / Mg²⁺
31,00 €
HEPES Buffered Saline Solution, 30 ml
Cat No
C-40000
HEPES Buffered Saline Solution (30 mM) available in different sizes.
12,00 €