How should I thaw the PromoCell normal human cells to obtain a high viability?
General protocol for anchorage‑dependent primary cells
Plate the diluted cell suspension directly at the recommended seeding density; Change the medium after 16–24 hrs
Option B (with centrifugation):
Centrifuge the cells, discard the supernatant, and resuspend the pellet in 1 ml of fresh medium.
Plate the cells at the recommended seeding density and change the medium no earlier than 24 hours after seeding.
For cell‑type‑specific details, refer to the Manual of the respective cell type.
- Remove the cryovial from liquid nitrogen and transport it on dry ice to the cell culture laboratory.
- Thaw the cells for 2 minutes at 37 °C until the contents are just defrosted. During thawing, keep the vial immersed in the water bath up to, but not above, the screw cap.
- Under a laminar flow hood, carefully disinfect the vial thoroughly with 70% ethanol.
- Transfer the thawed cell suspension into 9 ml of pre‑warmed culture medium (1:10 dilution).
- Proceed with one of the following options:
Plate the diluted cell suspension directly at the recommended seeding density; Change the medium after 16–24 hrs
Option B (with centrifugation):
Centrifuge the cells, discard the supernatant, and resuspend the pellet in 1 ml of fresh medium.
Plate the cells at the recommended seeding density and change the medium no earlier than 24 hours after seeding.
For cell‑type‑specific details, refer to the Manual of the respective cell type.
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