How can skeletal muscle cells (SkMC) be differentiated? Can you provide a protocol?

To differentiate skeletal muscle cells (SkMC) into myotubes, PromoCell recommends using early-stage cells with no more than 4-5 population doublings (PDs) after thawing.
 
Recommended differentiation workflow
1. Cell stage and expansion
- Use SkMC ideally at passage P2 or P3
- Cells should not exceed 4-5 population doublings
2. Growth phase
- Culture cells in Skeletal Muscle Cell Growth Medium (C‑23060)
- Grow cells to 60-80% confluency
3. Induction of differentiation
- Switch to serum‑free Skeletal Muscle Cell Differentiation Medium (C‑23061)
- This medium change induces differentiation and the formation of multinucleated syncytia (myotubes)
4. Maturation phase
- After 5 days, switch back to Skeletal Muscle Cell Growth Medium (C‑23060)
- Continue culture for an additional 8 days to complete differentiation