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How can high‑throughput organoid systems be set up? Are they limited to 96‑well formats?

Airway organoids such as bronchospheres can be cultured in 96‑well U‑bottom plates designed for suspension cells.
  • A commonly used example is 96‑well U‑bottom suspension plates (e.g. Greiner Bio‑One #650185).
  • A detailed protocol for culturing airway organoids in 96‑well plates is provided in the corresponding PromoCell Application Note "Generation of human airway organoids from primary cells". 
Plate formats not tested
384‑well plates and other commercially available plate formats have not been validated by PromoCell for this application.

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