For how long can monocyte‑derived dendritic cells be maintained in culture after differentiation is completed (day 7/8)?

Based on PromoCell’s experience, monocyte‑derived dendritic cells (DCs) can be maintained in culture for approximately 3 up to a maximum of 7 days after completion of differentiation (day 7/8 of the protocol).
 
Important considerations

• During this post‑differentiation period, cell morphology will gradually change
• Over time, the cells may appear increasingly degenerate
• Functional and phenotypic stability may therefore decline with prolonged culture

For optimal experimental results, it is recommended to use the cells as soon as possible after differentiation is completed. Additional guidance is provided in the Application Note "Generation of Monocyte-derived Dendritic Cells (moDCs)".