Does PromoCell offer a 3‑D system to prevent de‑differentiation or special culture conditions to stabilize the chondrocyte phenotype?
PromoCell Human Chondrocytes (HCH) can be expanded in standard monolayer culture using PromoCell Chondrocyte Growth Medium (C‑27101).
This medium is an optimized formulation supplemented with 10 % FBS and is suitable for efficient cell expansion.
De‑differentiation during monolayer culture
De‑differentiation of chondrocytes is a well‑known and unavoidable phenomenon during in vitro monolayer culture and typically occurs after approximately 2 weeks.
Despite this limitation, monolayer culture is necessary to expand chondrocytes to sufficient cell numbers.
Re‑differentiation using 3‑D culture systems
Once an adequate cell number has been obtained, the monolayer system can be replaced by a more complex 3‑D culture approach, such as:
PromoCell position
PromoCell does not offer a proprietary 3‑D culture system for chondrocytes.
However, 3‑D spheroid culture combined with Alcian Blue staining is used internally by PromoCell to characterize chondrocytes during quality control.
This procedure is very similar to the protocol used for chondrogenic differentiation of MSCs.
This medium is an optimized formulation supplemented with 10 % FBS and is suitable for efficient cell expansion.
De‑differentiation during monolayer culture
De‑differentiation of chondrocytes is a well‑known and unavoidable phenomenon during in vitro monolayer culture and typically occurs after approximately 2 weeks.
Despite this limitation, monolayer culture is necessary to expand chondrocytes to sufficient cell numbers.
Re‑differentiation using 3‑D culture systems
Once an adequate cell number has been obtained, the monolayer system can be replaced by a more complex 3‑D culture approach, such as:
- Culture in alginate beads or gels
- Use of degradable polymer scaffolds
- 3‑D spheroid culture
PromoCell position
PromoCell does not offer a proprietary 3‑D culture system for chondrocytes.
However, 3‑D spheroid culture combined with Alcian Blue staining is used internally by PromoCell to characterize chondrocytes during quality control.
This procedure is very similar to the protocol used for chondrogenic differentiation of MSCs.
