Does PromoCell determine the percentage of HDLECs in its HDMEC lots?

No. PromoCell does not routinely determine the ratio of Human Dermal Lymphatic Endothelial Cells (HDLEC) and Human Dermal Blood Endothelial Cells (HDBEC) within its HDMEC lots.
Based on our experience, the proportion of lymphatic endothelial cells is highly lot-dependent and can vary considerably, typically ranging from approximately 10% to 80%.
In general, juvenile HDMEC (C‑12210) tend to contain a lower proportion of HDLECs, whereas adult HDMEC (C‑12212) typically show a considerably higher proportion of lymphatic endothelial cells.
For applications requiring a pure endothelial cell population, PromoCell also offers Human Dermal Blood Endothelial Cells (HDBEC; C‑12211, C‑12225) and Human Dermal Lymphatic Endothelial Cells (HDLEC; C‑12216, C‑12217), which are derived from HDMEC cultures and subsequently enriched for either blood or lymphatic endothelial cells, respectively.