Do PromoCell keratinocytes require feeder cells?

No. PromoCell normal human epidermal keratinocytes (NHEK) do not require feeder cells when cultured in:

Keratinocyte Growth Medium 2 (C‑20011), or
Keratinocyte Growth Medium 3 (C‑20021).

Under these conditions, the keratinocytes grow as a monolayer in standard tissue‑culture‑treated flasks or dishes, without the need for any feeder layer.

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Normal Human Epidermal Keratinocytes (NHEK) juvenile foreskin, single donor

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Primary Human Keratinocytes isolated from the epidermis of juvenile foreskin or adult skin from single or pooled donors.

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Primary Human Keratinocytes isolated in serum-free and BPE-free Keratinocyte Growth Medium 3. Available from juvenile foreskin or adult skin from single or pooled donors.

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Primary Human Keratinocytes isolated in serum-free and BPE-free Keratinocyte Growth Medium 3. Available from juvenile foreskin or adult skin from single or pooled donors.

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Normal Human Epidermal Keratinocytes (NHEK) juvenile foreskin, isolated in Growth Medium 3, pooled

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Primary Human Keratinocytes isolated in serum-free and BPE-free Keratinocyte Growth Medium 3. Available from juvenile foreskin or adult skin from single or pooled donors.

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Normal Human Epidermal Keratinocytes (NHEK) adult, isolated in Growth Medium 3, pooled

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Primary Human Keratinocytes isolated in serum-free and BPE-free Keratinocyte Growth Medium 3. Available from juvenile foreskin or adult skin from single or pooled donors.

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