Do HUVECs grow in all your Endothelial Cell Growth Media?

Yes. In addition to the standard Endothelial Cell Growth Medium (C‑22010) and Endothelial Cell Growth Medium 2 (C‑22011), PromoCell HUVECs (single donor, pooled, and pre-screened) can also be cultured in:

• Endothelial Cell Growth Medium MV (C‑22020)
• Endothelial Cell Growth Medium MV2 (C‑22022)

While Endothelial Cell Growth Medium (C‑22010) remains the recommended standard medium for HUVEC culture, all four media support HUVEC growth. In general, low‑serum culture conditions are preferred for endothelial cells. Endothelial Cell Growth Medium (C‑22010) and Endothelial Cell Growth Medium 2 (C‑22011) therefore contain 2% FBS, whereas Endothelial Cell Growth Medium MV (C‑22020) and MV2 (C‑22022) contain 5% FBS. The choice of medium depends on the specific requirements of the application and the desired growth factor composition.
 
Please note: HUVEC 2 cell types (single donor HUVEC 2, C‑12206, and pooled HUVEC 2, C‑12208) are isolated directly in Endothelial Cell Growth Medium 2 and are therefore best cultured in Endothelial Cell Growth Medium 2 (C‑22011).