Do cryopreserved monocytes (C‑12909) attach and proliferate in culture?

Monocytes do not proliferate significantly in culture.
When cryopreserved monocytes (C‑12909) are thawed and cultured in Mononuclear Cell Medium (C‑28030), the cells largely remain in suspension.
In contrast, freshly isolated PBMC or monocytes rapidly attach when cultured in Monocyte Attachment Medium (C‑28051), typically within 1–1.5 hours. This enables efficient enrichment of the monocyte fraction through adherence.

Please note: In our experience, cryopreserved/thawed monocytes do not attach efficiently in Monocyte Attachment Medium and are therefore not suitable for adherence-based monocyte enrichment.