Can you control size / size distribution of organoids?
Description
Possibly. The size of the organoids may depend on the access to nutrients in the gel. For reference;Â https://pubmed.ncbi.nlm.nih.gov/31173716/
For best results we recommend the following:
- Avoid nutrient gradients. We recommend using a small; drop-like BME gel bead and high volumes of medium. Change the medium every day as we’ve observed that less frequent medium changes results in yellow culture medium indicative of a pH shift likely from an increase in cellular metabolism.
- For optimal cell distribution; it is important to control the “gelling” or setting of the ECM gel. Therefore; the temperature is critical! BME is liquid in the cold (4°C) and solidifies at 37°C. The gelling must be done in a very short time; otherwise the cells in the BME would sink to the bottom.