Are there any differences in the cultivation protocol when cultivating MSCs compared to other cell types?

Yes; there are a few differences: - We recommend replacing the MSC Growth Medium XF (C-28019) 3-4 h after seeding; as opposed to 16-24 hours after seeding for most other cell types/growth media. - When MSC Growth Medium XF; MSC Neurogenic Differentiation Medium; MSC Adipogenic Differentiation Medium 2 or MSC Osteogenic Differentiation Medium are used; flasks have to be coated with 10 µg/cm² (human or bovine) fibronectin according to the instruction manual. - We strongly recommend using Accutase (C-41310) for cell detachment instead of Trypsin. If Trypsin is used; contact time should not exceed 2 min.