Which medium does PromoCell recommend for endothelial cell transfection ?

There are several factors that can influence successful transfections; e.g. viability and density of the cells; choice of the transfection reagent; quality and type of the transfected molecules (plasmids; siRNA; oligonucleotides); as well as the culture medium and supplements used. When using PromoCell Endothelial Cell Growth Media for cell transfection; please follow the instructions below: Heparin; which is included in our Endothelial Cell Growth Medium (C-22010/C-22110); Medium 2 (C-22011/C-22111) and Medium MV (C-22020/C-22120) may reduce the transfection efficiency.  We therefore recommend to use our heparin-free Endothelial Cell Growth Medium MV2 (C-22022/C-22121). Alternatively; you may use Endothelial Cell Growth Medium Kit (C-22110); Medium 2 Kit (C-22111); or Medium MV Kit (C-22120) without adding the ECGS/heparin supplement to the Basal Medium. Please note: Before and after transfection; the cells should be cultured in complete Growth Medium including heparin to ensure optimal growth.