How do I best isolate RNA from PromoCell cell pellets (C-14**)?

There are two options for isolating RNA from cells stored in RNAlater Solution: 1) The solution is removed from the cells prior to extraction by centrifugation at 5;000 x g for 10 minutes at 4°C. Note: Because of the density of RNAlater® solution; greater centrifugal forces are required to spin down the cells. 2) If no pellet is visible after centrifugation; RNA can also be purified directly from the RNAlater® solution. This can be done by adding 2 ml of 10x lysis buffer; and proceeding normally.