Can airway organoid orientation (inward vs. outward) be controlled?
Yes. Airway organoid orientation can be influenced by the use or absence of an extracellular matrix (ECM).
- Without ECM, organoids tend to show a higher proportion of outward‑oriented ("apical-out") polarity.
- A published approach describes generating organoids initially embedded in ECM, followed by ECM dissociation and re‑seeding of free organoids in suspension without ECM, which promotes outward orientation. The protocol is described here.
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