What is the difference between NHEK.f and NHEK.f pooled?
Both NHEK.f and NHEK.f pooled are Normal Human Epidermal Keratinocytes derived from juvenile foreskin, but they differ in their donor composition and preparation.
NHEK.f single donor (C-12001)
NHEK.f single donor (C-12001)
- Isolated from the foreskin of a single donor
- Donor age range: 1 - ~10 years
- Cells reflect the biological characteristics of one individual donor
- After thawing, cells are at passage 2 (P2)
- Prepared from the foreskins of two or three individual donors
- Cells from each donor are expanded separately in individual tissue‑culture vessels, and pooled after secondary culture, prior to cryopreservation
- Pooling reduces donor‑to‑donor variability
- After thawing, cells are also at passage 2 (P2)
- NHEK.f: single‑donor cells → suitable for donor‑specific studies
- NHEK.f pooled: multi‑donor pool → suitable for more standardized and robust experiments
Products relevant to this topic
Normal Human Epidermal Keratinocytes (NHEK) juvenile foreskin, single donor
Cat No
C-12001
Primary Human Keratinocytes isolated from the epidermis of juvenile foreskin or adult skin from single or pooled donors.
Normal Human Epidermal Keratinocytes (NHEK) juvenile foreskin, pooled
Cat No
C-12005
Primary Human Keratinocytes isolated from the epidermis of juvenile foreskin or adult skin from single or pooled donors.