Do I need additional supplements when working with PromoCell speicalized media? Do I have to add extra FBS?
No. After adding the SupplementMix or SupplementPack to the corresponding PromoCell basal medium, you obtain a complete culture medium.
No additional supplementation with fetal calf serum, growth factors, or L-glutamine is required, as all components are already present at optimized concentrations.
Important note on antibiotics
No additional supplementation with fetal calf serum, growth factors, or L-glutamine is required, as all components are already present at optimized concentrations.
Important note on antibiotics
- PromoCell media do not contain antibiotics.
- If desired, penicillin/streptomycin or gentamicin/amphotericin B can be added at standard concentrations.
- Please note that the use of antibiotics may reduce cell doubling time by up to 20%.
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