What type of pre-coated culture vessels should I use for primary endothelial cells?

PromoCell Endothelial Cell Growth Media have been developed to support the optimal growth of primary endothelial cells on standard tissue culture-treated (TC-treated) plasticware. Therefore, pre-coating of culture vessels is generally not required.
If pre-coated culture vessels are preferred, suitable coating materials include:

• Collagen type I
• Gelatin
• Fibronectin

Please note: The choice of coating material can influence cell attachment, morphology, and gene expression. To ensure comparable results, the same coating material and culture conditions should be used consistently throughout a study.